In: Sydowia 77, (2025): 291-301; ISSN 0082-0598, DOI 10.12905/0380.sydowia77-2025-0291, Published online on December 12, 2024

Phosphate and potassium release dynamics of two root endophytic aquatic hyphomycetes from Kumaun Himalaya, India

Suresh C. Sati, Anjali Koranga Gashyal,*, Prabha Pant & Kapil Khulbe

1 Aquatic Myco-pathology lab, Department of Botany, DSB Campus, Kumaun University, Nainital, Uttarakhand, India
2 ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora-263001, Uttarakhand, India
* e-mail: anjalikoranga56@gmail.com

Sati S.C., Gashyal Koranga A., Pant P. & Khulbe K. (2025) Phosphate and potassium release dynamics of two root endophytic
hyphomycetes from Kumaun Himalaya, India. – Sydowia 77: 291–301.

Two root endophytic aquatic hyphomycetes, viz. Flagellospora penicillioides and Varicosporium elodeae, isolated from the
roots of Ageratina adenophora and Eurya acuminata growing in ravine areas of Kumaun Himalayan region, Uttarakhand (India),
were assessed for their phosphate and potassium solubilization efficiency in vitro. The quantitative evaluation of phosphate and
potassium solubilization was carried out using spectrophotometric analysis by growing them on Pikovskaya and Aleksandrov
broth mediums. Simultaneously, the pH value and mycelial-dry-weight were also measured at intervals of 7, 14, and 21 days after
inoculation. The P and K solubilization ef?ciency of F. penicillioides and V. elodeae showed the maximum solubilization 21days
after inoculation (1.186 mg P/l and 66.484 mg K/l as well as 0.877 mg P/l and 58.309 mg K/l, respectively). A subsequent increment
in mycelial weight and a gradual decrease in pH with a growing incubation period were also recorded in the respective
broth cultures by both fungal isolates. The isolated fungal strains were found to be potent phosphate and potassium solubilizers
which can be used as biofertilizers for sustainable agricultural practices. To the best of our knowledge the present work is the
first report on F. penicillioides and V. elodeae as P and K solubilizer.

Keywords: Aquatic hyphomycetes, Flagellospora penicillioides, Varicosporium elodeae, endophyte, phosphate, potassium solubilization.