In: Phyton 61 (2021): S. 97-107; DOI: 10.12905/0380.phyton61-2021-0097; Published online on 22 December 2021
Molecular systematics of selected species of family Boraginaceae based on the rps14 gene
Tasmeena Shahzad , Wasim Akhtar & Tariq Mahmood
with 6 figures and 5 tables
Key words: Boraginaceae, phylogeny, evolutionary relationship, chloroplast genome, rps14 gene.
Shahzad T., Akhtar W. & Mahmood T. 2021. Molecular systematics of selected species of family Boraginaceae based on the rps14 gene. – Phyton (Horn, Austria) 61: 97–107, with 6 figures and 5 tables.
Various molecular markers have been used among selected taxa of Boraginaceae, but this is the first time that evolutionary relationship, protein conformation analyses and their validation through Ramachandran plots of selected species of Boraginaceae were examined using the chloroplast rps14 gene. Analysis of three phylograms reveals low genetic distance of 0.0100 that indicates close genetic kinship among the 15 species studied. Phylogenetic trees revealed that Paracaryum himalayense is the earliest evolved species and ancestral to all other selected species. The 3D protein structural models for rps14 protein sequences were predicted through I-TASSER. Models with highest C-score were selected for Ramachandran plot analysis. For some of the selected species, we obtained structural models of very high quality as they had < 2 % amino acid residues occurring in the outlier region.