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Phyton Vol. 55/2 E-Book S 233-249
In Vitro Multiplication from Seeds and Adult Explants of two Ornam
Artikel Nr 2634
Preis 11,00
In: Phyton 55, Fasc. 2 (2015): S. 233-249 DOI: 10.12905/0380.phyton55(2)2015-0233

In Vitro Multiplication from Seeds and Adult Explants of two Ornamental Ruellia (Acanthaceae) species of Brazilian Savanna (Cerrado)

Key words: Ruellia, sustainability, native species, in vitro germination, sub­shrubs.

Lima M. R., Pereira L. A. R. & Silveira C. E. S. 2015. In vitro multiplication from seeds and adult explants of two ornamental Ruellia (Acanthaceae) species of Bra­zilian Savanna (Cerrado). – Phyton (Horn, Austria) 55 (2): 233–249, with 3 figures.

The Cerrado has wide flora diversity; however, this biome has been devastated due to agropecuary activities. The objective of this work was to provide a multipli­cation protocol from in vitro seed germination and adult explants of two Cerrado species, Ruellia nitens and Ruellia incompta, with high potential as ornamentals. The seeds of both species were decontaminated and inoculated in water-agar, half and full strength MS media. In general, R. nitens presented lower decontamition values (> 50%) than R. incompta (~100%). Independently of salt content, R. nitens seedlings in MS medium showed larger shoots and roots. R. incompta shoot/root lengths were not affected by the culture media. Seedling development was similar for both species up to the 30th day of culture. Nevertheless, 30-day old R. incompta seedlings started presenting tissue oxidation and lenticels in the roots and died around the 60th day. The use of adult material for multiplication was only successful for R. nitens. These explants were decontaminated with hypochlorite based solu­tions. Although, Ca-hypochlorite resulted in lower disinfecting rate, the explant survival was much better than those of explants treated with Na-hypochlorite. While, in no hormone medium the explant elongated better, media containing BAP induced better sprouting rate. The results support the use of in vitro techniques as a viable way for the propagation of Cerrado species, which is crucial for the conser­vation of this highly threatened biome.