In: Phyton 62-63 (2023): S. 87-96; DOI: 10.12905/0380.phyton62-63-2023-00787 Published online on 31 March 2023

Transfer of the Arabidopsis CBF1, a cold-induced transcription activator, into Phalaenopsis via pollen-tube pathway

Ren Rui Wang, Lan Yu, Ping Kuang, Shi Lin Su, Feng Xia Mo, Xi Ruan, Li Juan Niu & Jie Li

with 11 figures
Key words: Phalaenopsis, AtCBF1 gene, pollen-tube pathway, cold tolerance.

Summary
Wang R. R., Yu L., Kuang P., Su S. L., Mo F. X., Ruan X., Niu L. J. & Li J. 2023. Transfer of the Arabidopsis CBF1, a cold-induced transcription activator, into Phalaenopsis via pollen-tube pathway. – Phyton (Horn, Austria) 62–63: 87–96, with 11 figures.
To elucidate the effects of expression of a cold-induced transcription activator (the C-repeat binding factor of Arabidopsis thaliana, AtCBF1) on cold-tolerance in Phalaenopsis, the plasmid vector pBI121-AtCBF1 was successfully transferred into Phalaenopsis via the pollen-tube pathway. The plasmid also included a kanamycin resistance gene, and the resulting kanamycin-resistant transgenic plants were cultured and subsequently tested for the presence of the AtCBF1 gene and for cold-tolerance. The CBF1 specific band of 660 bp was detected by PCR and RT-PCR, and differences in AtCBF1 gene expression among four selected transgenic lines were revealed by RT-qPCR analysis. Under low temperature stress, the chilling injury symptoms in leaves of transgenic lines were significantly slighter than those of the control plantlets. The results indicated that the AtCBF1 gene was expressed in Phalaenopsis, and the capacities of low temperature stress tolerance in the transgenic plants were improved.