In: Sydowia 73, (2021): 209-2015; ISSN 0082-0598, DOI 10.12905/0380.sydowia73-2021-0209, Published online on February 26, 2021

Yield potential and antioxidant activity of Indian Ganoderma strains

Vedukola PullaReddy, Dharmesh Gupta, Sudheer Kumar Annepu, Anupam Barh, Satish Kumar,
Manisha Thakur & Joginder Pal

Department of Mycology and Plant Pathology, Dr Yashwanth Singh Parmar University of Horticulture and Forestry,
Nauni, Solan, India

ICAR-Directorate of Mushroom Research, Chambaghat, Solan (Himachal Pradesh), India
* e-mail: pullareddyvedukola@gmail.com

PullaReddy V., Gupta, D., Kumar Annepu S., Barh A., Kumar S., Manisha Thakur M. & Pal, J. (2021) Yield potential and antioxidant
activity of Indian Ganoderma strains. – Sydowia 73: 209–215.

The cultivation of Reishi or Lingzhi mushroom using artificial bag log method has been optimized for four different strains
viz., DMRO-44, DMRO-45, DMRO-90 and DMRO-207. Various lignocellulosic materials were tested as growing substrate, and
the sawdust of Toona celiata supplemented with wheat bran resulted in maximum Biological Efficiency (BE) of 22.10±1.01 % for
the strain DMRO-90. The free radical scavenging activity of the fruiting bodies were investigated by DPPH (1,1-diphenyl-
2-picrylhydrazyl), ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] and reducing power assay. Acetonic extracts
at a concentration of 1000 µg/ml showed significantly maximum radical scavenging activity for all the four strains. At the concentration
of 1000 µg/ml, the strain DMRO-90 exhibited the highest DPPH scavenging activity of 67.05 % and ABTS scavenging
activity was recorded as 66.05 %. Preliminary biochemical analysis identified the presence of alkaloids, carbohydrates, flavonoids,
phenolic compounds, terpenoids and tannins in all the four strains. The quantitative estimation revealed that, strain
DMRO-90 significantly contains higher total phenolics (50.02 mg GAE/g), ß-carotene (0.89 µg/g) and lycopene (0.36 µg/g). This
study indicated the scope for mass production of Ganoderma mushroom on sawdust substrate and the fruiting bodies can act as
a potential source for preparation of dietary supplements and mushroom fortified functional foods.

Keywords: Reishi, medicinal mushrooms, growing substrate, DPPH, ABTS, reducing power assay.