In: Sydowia 77, (2025): 111-118; ISSN 0082-0598, DOI 10.12905/0380.sydowia77-2025-0111, Published online on September 10, 2024

Biodiversity analysis of arbuscular mycorrhizal fungal association with Euphorbiaceae

Mosa Ayshasiddeka, Mohammad Majidul Islam, Hanif Ali, Mohammad Redwanul Haque, Kahkashan Perveen, Alanoud T. Alfagham, Riyaz Z. Sayyed,* & Tanzima Yeasmin,*

1 Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh
2 Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh
3 Department of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia
4 Asian PGPR Society for Sustainable Agriculture, Auburn Ventures, Auburn, Alabama, U.S.A.
* e-mails: sayyedrz@gmail.com, yeasmin_bio@yahoo.com

Ayshasiddeka M., Islam M.M., Ali H., Haque M.R., Perveen K., Alfagham A.T., Sayyed R.Z. & Yeasmin T. (2024) Biodiversity
analysis of arbuscular mycorrhizal fungal association with Euphorbiaceae. – Sydowia 77: 111–118.

Mycorrhizae have been recognized as plant growth-promoting microorganisms since ancient times. However, their association with host plants is influenced by various factors, including the species of the host plant and the physicochemical properties of the soil. Determination of such factors will help in their sustainable and effective use as bioinoculants. This study aimed to evaluate mycorrhizal status and select plants for mycorrhizal inoculum preparation. To explore this relationship, a study was conducted to determine the arbuscular mycorrhizal association in the Euphorbiaceae on the Rajshahi University Campus in Bangladesh. Root samples of 25 different plants were stained with trypan blue and observed microscopically. The roots of 19 plants showed good mycorrhizal colonization, in the range of 25 %±2.65 to 98 %±1.00, while the roots of the remaining six plants did not show mycorrhizal colonization. The highest root colonization was observed in the Acalypha indica (98 %±1.00) plant as against the lowest root colonization observed in Euphorbia milli (25 %±02.65). Glomus mosseae, G. dimorphicum, G. geosporum, and Entrophospora colombinana were grown on solid agar media. The frequencies of mycorrhizal infection were strong and significantly correlated with soil pH and moisture. The study found a strong positive correlation between mycorrhizal association and soil pH and moisture content. A basic soil pH (8.4±0.023) and high moisture content (21.00 %±6.86) of rhizospheric soil supported the highest root colonization in A. indica (98 %±1.00) compared to neutral pH (7.40±0.092) and low moisture content (10.80 %±1.97) with the affected root colonization in E. milli. As an essential tool to manage the nutritional status, AM fungal technology can reduce environmental pollution. The results suggest that Acalypha indica could serve as an initial natural source of arbuscular mycorrhizal fungi and could be used as a stock plant for mycorrhizal inoculum in future applications.

Keywords: Arbuscular mycorrhiza, colonization, Euphorbiaceae, spore, mycorrhizal inoculum.