In: Sydowia 71, (2019): 0011-0015, ISSN 0082-0598, DOI 10.12905/0380.sydowia71-2019-0011, Published online on May 13, 2019

Decolorization and simultaneous electricity generation from aquatic pharmaceutical malachite green using Shiitake mushroom

Pimprapa Chaijak, Chikashi Sato, Noris Paucar, Chontisa Sukkasem & Monthon Lertworapreecha

Chaijak P., Sato C., Paucar N., Sukkasem C. & Lertworapreecha M. (2005) Decolorization and simultaneous electricity generation from aquatic pharmaceutical malachite green using Shiitake mushroom. – Sydowia 71: 11–15.

Shiitake mushroom (Lentinula edodes) is a valuable mushroom found around the world. This study investigated: (a) the activities of extracellular oxidoreductase enzymes, specifically laccase (Lac) and manganese peroxidase (MnP), produced by L. edodes NSLE1; (b) the decolorization potential of Lac and MnP using 150 mg l–1 malachite green (MG); and (c) the MG decolorization and simultaneous electricity generation in a single-chamber microbial fuel cell (sMFC). Results showed that L. edodes NSLE1 secreted Lac and MnP at the maximum activities of 52.66±1.97 and 52.16±1.03 U ml–1, respectively. The maximum color removal potential in the in vitro experiment was 69.45±2.51 %. The color removal was enhanced (75.26±0.47 %) in the sMFC while yielding the maximum open circuit voltage of 473.63 mV. The results indicated that L. edodes NSLE1 has the potential to treat wastewater contaminated with MG dye and generate electricity simultaneously. No previous studies have shown MG removal using a sMFC with Shiitake mushroom, while simultaneously generating electricity

Keywords: carcinogen, dye removal, laccase, manganese peroxidase, mushroom, aquatic pharmaceutical